公鸡弱精症相关候选基因的表达分析

doi:10.11843/j.issn.0366-6964.2015.06.003

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (6): 889-895.doi: 10.11843/j.issn.0366-6964.2015.06.003

公鸡弱精症相关候选基因的表达分析

富丽^1，2，孙研研²，薛夫光²，刘冉冉²，白皓²，常国斌¹，陈国宏¹，陈继兰^2*

富丽(1988-)，女，山东烟台人，硕士，主要从事家禽分子遗传研究，Tel：010-62816005，E-mail：momomook@126.com

收稿日期:2014-08-15 出版日期:2015-06-23 发布日期:2015-06-23
通讯作者: 陈继兰，研究员，E-mail：Chen.jilan@163.com
作者简介:富丽(1988-)，女，山东烟台人，硕士，主要从事家禽分子遗传研究，Tel：010-62816005，E-mail：momomook@126.com
基金资助:
国家自然科学基金（31372304）；中国农业科学院科技创新工程（ASTIP-IAS04）

The Expression of the Six Candidate Genes for Asthenospermia of the Rooster

FU Li^1，2，SUN Yan-yan²，XUE Fu-guang²，LIU Ran-ran²，BAI Hao²，CHANG Guo-bin¹，CHEN Guo-hong¹，CHEN Ji-lan^2*

（1.扬州大学动物科学与技术学院，扬州 225009；2.中国农业科学院北京畜牧兽医研究所，农业部畜禽资源与种质创新重点实验室，北京 100193）

Received:2014-08-15 Online:2015-06-23 Published:2015-06-23

摘要/Abstract

摘要：

为了探究公鸡弱精症的发病机理，本研究对前期应用数字基因表达谱技术(Digital gene expression，DGE)及生物信息学分析得到的与公鸡弱精症相关的6个候选基因(COX7B、PTGDS、hPGDS、SPAG6、WNT2、CCNF)进行表达分析。选取300只42周龄的北京油鸡公鸡，进行为期4周的精液品质检测，从中筛出弱精及正常个体各15只。采用RT-qPCR对这6个候选基因在两组个体的睾丸组织的表达量进行相对定量检测。结果发现，COX7B、PTGDS在弱精组个体睾丸的表达量显著高于正常个体(P<0.01)，WNT2、hPGDS、CCNF在弱精组个体的表达量显著低于正常个体(P<0.05)，SPAG6在两组睾丸中的表达量没有显著差异，该结果与DGE的结果相吻合。通过DGE筛选出与弱精症相关的6个候选基因的表达模式在本研究的群体中得到进一步验证，可作为公鸡弱精症的重要候选基因，进一步进行功能分析与验证，为揭示公鸡弱精症的发病机理提供依据。

Abstract:

To explore the pathogenesis of the rooster asthenospermia，the expression profiles of 6 candidate genes (COX7B，PTGDS，hPGDS，SPAG6，WNT2 and CCNF) selected from the previous digital gene expression profiling (DGE) and bioinformatics analysis，were analyzed.A total of 300 Beijing-You roosters of 42 weeks were detected for 4 weeks for semen quality including semen volume，sperm mobility，etc.Fifteen asthenospermia and 15 normal roosters were selected accordingly.Real-time quantitative PCR were performed to determine the expression of the 6 genes in the testicular of those roosters.The results indicated that COX7B and PTGDS were significantly high-expressed in the asthenospermia group (P<0.01)，while the WNT2，hPGDS and CCNF were significantly low-expressed in the asthenospermia group (P<0.05).However，the expression of SPAG6 gene was not significantly different in the 2 groups.These results were in accordance with the previous DGE analysis.The expression patterns of the 6 genes identified from DGE for asthenospermia in chickens were validated in the present study.They can be important candidate genes for further functional study and revealing the mechanism of chicken asthenospermia.

中图分类号:

S831
S813.3

富丽，孙研研，薛夫光，刘冉冉，白皓，常国斌，陈国宏，陈继兰. 公鸡弱精症相关候选基因的表达分析[J]. 畜牧兽医学报, 2015, 46(6): 889-895.

FU Li，SUN Yan-yan，XUE Fu-guang，LIU Ran-ran，BAI Hao，CHANG Guo-bin，CHEN Guo-hong，CHEN Ji-lan. The Expression of the Six Candidate Genes for Asthenospermia of the Rooster[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 889-895.

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公鸡弱精症相关候选基因的表达分析

The Expression of the Six Candidate Genes for Asthenospermia of the Rooster

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